Phytochemical analysis, antioxidant and antimicrobial studies of fruit rind of Couroupita guianensis (AUBL)

Couroupita guianensis possesses antibiotic, antifungal, antiseptic and analgesic qualities). The present work was undertaken to know more about its phytochemical analysis, compound isolation antimicrobial activity and antioxidant status to ensure its usage in Pharmaceutical industries. The different solvent extracts were loaded on cut wells with the volume of 100ul of pre-sterilized medium of each petriplate containing swabbed pathogens such as Staphylococcus aureus, E.coli, Micrococcus, Cornybacterium diptheria, Candida albicans, were incubated and observed zone of inhibition rate and determined MIC. Phytochemical analysis were carried out by TLC and HPLC methods and antioxidant activity by 1,1-diphenyl-2picryhydrazyl. chloroform, hexane and ethanol extract of fruit rind of Couroupita guianensis Aubl. showed its significant antibacterial and antifungal activity at the various conc. against major microbes. Qualitative phytochemical observation showed main components like Rutin,Quercetin, Kaempherol, Farmaricetin, Lutolin, Ursolic acid Saponines derivatives like Hopanes, which have anticancer activity. Number of molecules of DPPH scavenged per molecule of the tested compounds was found to be the strongest antiradical activity.